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首頁 > 核酸生物學(xué) > 核酸電泳 > 雙鏈DNA Marker

D2000 DNA ladder(100-2000bp)

D2000 DNA ladder(100-2000bp)

產(chǎn)品編號(hào):RTM415-02

產(chǎn)品規(guī)格:100次

數(shù)量
價(jià)格 ¥150

D2000 DNA Ladder

 

● 產(chǎn)品編號(hào)及規(guī)格:

    RTM415-02       100T(2×250μl)       150元

 

● 產(chǎn)品簡(jiǎn)介:

本產(chǎn)品是由6條帶狀雙鏈DNA條帶組成,適用于瓊脂糖凝膠電泳中DNA條帶的分析。產(chǎn)品中已含有1×loading buffer,可根據(jù)實(shí)驗(yàn)需要,直接取2-5μl電泳,使用方便,電泳圖像清晰。6條帶的大小分別為100250,500,750,10002000bp。其中750bp條帶為100ng/5μl,顯示加亮帶,其余條帶濃度50ng/5μl。


● 使用方法:

1. 2-5μl本產(chǎn)品加入到瓊脂糖凝膠的加樣孔中(每1mm×1mm加樣孔加1μl,如果加樣孔寬于

5mm,可以適當(dāng)增加上樣量)就行電泳。

2. 建議電泳條件:凝膠濃度為1.5-2.0%,凝膠長(zhǎng)度5-7cm,電泳電壓4-10v/cm,電泳時(shí)間15-20

分鐘。

3. 通過EB染色后紫外燈下觀察條帶。

注:如果使用Goldview等染料就行染色,由于靈敏度比EB低,請(qǐng)酌情增加Marker的上樣量。

● 注意事項(xiàng):

1. 瓊脂糖的質(zhì)量對(duì)DNA的電泳有很大影響,電泳時(shí)請(qǐng)盡量使用質(zhì)量?jī)?yōu)等的瓊脂糖。

 

2. 請(qǐng)使用新鮮配制的電泳緩沖液和新鮮配制的瓊脂糖凝膠進(jìn)行電泳,以保證Marker良好的分離效果。

使用RTM415  D2000 DNA ladder產(chǎn)品發(fā)表部分文章列表:

1. [2021 IF=20.37] A multi-locus phylogenetic evaluation of Diaporthe (Phomopsis)

Marker :RTM415,D2000 DNA ladder

Author: Dhanushka Udayanga & Xingzhong Liu &Pedro W. Crous & Eric H. C. McKenzie &Ekachai Chukeatirote & Kevin D. Hyde

Journal: Fungal Diversity (2012) 56:157–171

InstitutionState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences

Paper linkhttps://link.springer.com/article/10.1007/s13225-012-0190-9

 

2. [2021 IF=1.17] Rhopalostroma brevistipitatum sp. nov. from Thailand with an extended generic description for Rhopalostroma.

Marker :RTM415,D2000 DNA ladder

Author: DINUSHANI A. DARANAGAMA, XINGZHONG LIU, SUNITA CHAMYUANG, MARC STADLER,ALI H.BAHKALI, KEVIN D. HYDE

Journal: Phytotaxa2015,227 (3): 229–242

InstitutionState Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences

Paper linkhttps://www.biotaxa.org/Phytotaxa/article/view/phytotaxa.227.3.2/0

 

3. [2021 IF=3.24] Envelope Proteins ofWhite Spot Syndrome Virus (WSSV) Interact with Litopenaeus vannamei Peritrophin-Like Protein (LvPT).

Marker :RTM415,D2000 DNA ladder

Author: Shijun Xie, Xiaojun Zhang, Jiquan Zhang, Fuhua Li, Jianhai Xiang

Journal: Plos One,Published: December 21, 2015

InstitutionKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences

Paper link https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0144922

 

4. [2021 IF=2.88] Genetic diversity and genetic structure of Decalobanthus boisianus in Hainan Island, China.

Marker :RTM415,D2000 DNA ladder

Author: Huan Jiang,Wenxing Long, Hui Zhang,Chengneng Mi,Tao Zhou,Zongzhu Chen

Journal: Ecology and Evolotion Volume 569, 15 March 2019, Pages 28-30

InstitutionInstitute of Tropical Agriculture and Forestry, Hainan University

Paper link https://doi.org/10.1002/ece3.5127

 

5. [2021 IF=1.17] Dendrostoma covidicola sp. nov. (Erythrogloeaceae, Diaporthales) on Fagus sylvatica from Sichuan Province, China.

Author: MILAN C. SAMARAKOON, ITTHAYAKORN PROMPUTTHA & JIAN-KUI (JACK) LI

Marker: RTM415,D2000 DNA ladder

Journal: Phytotaxa. 2021,483 (2): 085–094.

Institution:University of Electronic Science and Technology of China

Paper linkhttps://doi.org/10.11646/phytotaxa.483.2.1

 

6. [2021 IF=2.6] Natural occurrence of Alternaria mycotoxins in wheat and potential of reducing associated risks using magnolol.

Marker :RTM415,D2000 DNA ladder

Author: Dongmei Jiang, Dizhe Wei, Hongtao Li, Liuqing Wang, Nan Jiang,Yunchao Li and Meng Wang

Journal: Journal of the Science of Food and Agriculture Volume101, Issue7,2021,3071-3077

InstitutionBeijing Research Center for Agricultural Standards and Testing, Beijing Academy of Agriculture and Forestry Sciences

Paper linkhttps://onlinelibrary.wiley.com/doi/10.1002/jsfa.10901

 

7. [2021 IF=20.37] Taxonomy, Phylogeny, Molecular Dating and Ancestral State Reconstruction of Xylariomycetidae (Sordariomycetes)

Marker :RTM415,D2000 DNA ladder

Author: Kevin D Hyde,Jian-Kui Liu

Journal: Fungal Diversity volume 112, pages 1–88 (2022)

InstitutionSchool of Life Science and Technology, Center for Informational Biology, University of Electronic Science and Technology of China

Paper linkhttps://link.springer.com/article/10.1007/s13225-021-00495-5

 

8. [2022 IF=8.4] Target and acidic tumor microenvironment-induced in situ HCR for bispecific tumor cell imaging and therapy.

Marker :RTM415,D2000 DNA ladder

Author: Zihan Song, Yuzhe Fan, Su Zeng, Lianli Sun

Journal: Sensors and Actuators: B. Chemical  413 (2024) 135867

InstitutionCollege of Pharmaceutical Sciences, Zhejiang University

Paper linkhttps://doi.org/10.1016/j.snb.2024.135867

 

9. [2023 IF=14.3] Bioactive Self-Assembled Nanoregulator Enhances Hematoma Resolution and Inhibits Neuroin?ammation in the Treatment of Intracerebral Hemorrhage.

Marker :RTM415,D2000 DNA ladder

Author: Wenyan Yu, Chengyuan Che, Yi Yang, Yuzhen Zhao, Junjie Liu, Aibing Chen,and Jinjin Shi

Journal: Adv. Sci. 2024, 2408647

InstitutionSchool of Pharmaceutical Sciences,Zhengzhou University

Paper linkhttps://doi.org/10.1002/advs.202408647

 


 
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